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Participant 9

Centre National de la Recherche Scientifique (CNRS)

Partner 9:CNRS UMR 8576, «Unité de Glycobiologie Structurale et Fonctionnelle», (UGSF),Université des Sciences et Technologies Lille1, 59655 Villeneuve d’Ascq Cedex, France (Team leader/Responsible Scientist: Dr Jean-Claude Michalski) Key skills: Separation and structural analysis of oligosaccharides from tissues, serum and urine.

Laboratory background and technical information: The UGSF is a joint research unit between CNRS (Centre National de la Recherche Scientifique ) and Université des Sciences et Technologies de Lille1 (USTL). This institute is managed by Dr Jean-Claude Michalski, senior researcher employed by INSERM (Institut National de la Santé et de la Recherche Médicale). The laboratory has a long standing interest in the study of structure, biological functions and normal and pathological metabolism of glycoconjugates. Part of the projects developed in the institute concerns the study of the storage material in lysosomal diseases and the study of glycosidases specificities with a special interest for mannosidases involvement in the catabolism and processing of high-mannose type glycans. The group has also along experience in structural glycobiology such as the characterization of glycosylation profile of glycoproteins of biological or clinical interest. The laboratory possesses the equipment and personal allowing isolation and structural characterization of carbohydrates including HPLC, Dionex HPAEC, GC/MS, MALDI-MS, ESI-Ion-Trap MS, QTOF-MS, 400,600 and 800 MHz NMR.The laboratory has also proteomics and applied bioinformatics facilities.

Capacity to contribute to the project: The group has a long experience in structural glycobiology. It is also involved in the study of substrate specificity of cellular mannosidases and has contributed to the understanding of lysosomal and cytosolic mannosidases activities and biological functions. Our contribution will concern the quantification, fingerprinting and fine structural characterization of tissues and biological fluids oligosaccharides in different ERT conditions experiments. The group is also experienced in proteomics analysis. This approach will be used as a complement of the transcriptome analysis (Partner 5).

Members involved: To be supported by the project:One post-doctoral fellow structural analysis, proteomics (36 pm) The post-doc fellow will take in charge the separation of oligosaccharides isomers from biological samples (urine, serum) of the patients , perform the mass-spectrometry analysis(human biological fluids and animal tissues ) and contribute to the development and standardization of suitable and sensitive MS procedures applicable for the analysis of oligomannosides isomers. Other contributing members of the laboratory: Dr Jean-Claude Michalski ,PhD,director of the institute, supervision of structural analysis ,proteomics (4pm), Dr Willy Morelle,PhD, lecturer, Mass spectrometry analysis (6pm), Dr Frederic Chirat ,lecturer, HPLC, Capillary electrophoresis, Fluorophore Assisted Carbohydrate electrophoresis (6pm), Yves Leroy, CNRS Ingenior, GC/MS, HPAEC Dionex (6pm), Marie-Christine Slomianny, CNRS Ingenior , Mass spectrometry (8pm), Anne-Marie Mir CNRS Technician HPLC (6pm).

TOTAL EXPECTED EFFORT: 72 pm

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