Collaborative Research Centre 877

Proteolysis as a Regulatory Event in Pathophysiology

Christian-Albrechts-Universität zu Kiel

Education and Academic Positions
2007 Habilitation in Analytical Biochemistry, Saarland University Saarbrücken
1995-1999 Dissertation at the German Cancer Research Centre, Heidelberg & Saarland University, Saarbrücken
1989-1995 Studies of Chemistry at the Saarland University, Saarbrücken
Research Experience
2010 Invited Guest-Professor for Analytical Chemistry, Université Pau, France
Since 2008 W2-Professor for "Systematic Proteome Research" at the University of Kiel
2007-2008 Privatdozent, Institut für Technische Biochemie, Saarland University, Saarbrücken
2004 Research Asscociate, Laboratoire de la Spectrometrie de Masse Bio-Organique, Université Louis-Pasteur/CNRS, Strasbourg, France
1999-2007 Scientific Assistant, Institut für Technische Biochemie, Saarland University, Saarbrücken
Awards and Honors
Since 2011 Vice-Chair of the "Initiative on Model Organisms Proteomics (iMOP)" within the Human Proteome Organization (HUPO)
List of 5 Selected Publications
1. Cassidy L, Prasse D, Linke D, Schmitz RA, Tholey A (2016) Combination of bottom-up 2D-LC-MS and semi-top-down GelFree-LC-MS enhances coverage of proteome and low molecular weight short open reading frame encoded peptides of the archeon Methanosarcina mazei. J Proteome Res 15: 3773-3783
2. Hung CW, Klein T, Cassidy L, Linke D, Lange S, Anders U, Bureik M, Heinzle E, Schneider K, Tholey A (2016) Comparative Proteome Analysis in Schizosaccharomyces pombe Identifies Metabolic Targets to Improve Protein Production and Secretion. Mol Cell Proteomics 15: 3090-3106
3. Tucher J, Linke D, Koudelka T, Cassidy L, Tredup C, Wichert R, Pietrzik C, Becker-Pauly C, Tholey A (2014) LC-MS Based Cleavage Site Profiling of the Proteases ADAM10 and ADAM17 Using Proteome-Derived Peptide Libraries. J Proteome Res 13: 2205-2214
4. Broder C, Vadon-Le Goff S, Arnold P, Konerding M, Bahr K, Fisher LW, Müller S, Overall CM, Bond JS, Koudelka T, Tholey A, Hulmes DJS, Moali C, Becker-Pauly C (2013) Metalloproteases meprin α and meprin β are C- and N-procollagen proteinases important for collagen assembly and tensile strength. Proc Natl Acad Sci U S A 110: 14219-14224
5. Michalek M, Sönnichsen FD, Wechselberger R, Dingley AJ, Hung CW, Kopp A, Wienk H, Simanski M, Herbst R, Lorenzen I, Marciano-Cabral F, Gelhaus C, Gutsmann T, Tholey A, Grötzinger J, Leippe M (2013) Structure and function of a unique pore-forming protein from a pathogenic acanthamoeba. Nat Chem Biol 9: 37-42