IRTG libary for graduate students

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(2) Basic Methods in Protein Purification and Analysis
- A Laboratory Manual -

Edited by: RJ Simpson, PD Adams, and EA Golemis

This new, user-friendly manual contains essential step-by-step protocols for protein purification and analysis. Derived in large part from the popular laboratory manuals Protein and Proteomics, Purification Proteins for Proteomics (both by Richard Simpson), and Protein-Protein- Interaction (edited by Erica Golemis and Peter Adams), this new manual is designed for routine, day-to-day use at the bench. By integrating both established in vitro and in vivo molecular techniques with more modern in silico methods, this manual takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in charaterizing and identifying proteins, protein complexes, and protein-protein interactions. These step-wise methods are combined with troubleshooting advice and guidance on selecting an appropriate experimental strategy, making this new manual a handy yet informative resource for bench scientists with all levels of experience.