IRTG libary for graduate students

 

for lending of books contact the coordinator

(4) Molecular Cloning 1-3
- A Laboratory Manual -

Edited by J. Sambrook and DW Russel (2001)

Volume 1
1. Plasmids and Their Usefulness in Molecular Cloning
2. Bacteriophage lambda and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High-capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells

Volume 2
8. In vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13: Mutagenesis
14: Screening Expression Libraries

Volume 3
15. Expression of Cloned Genes in Escherichia coli
16. Introducing Cloned Genes into Cultured Mammalian Cells
17. Analysis of Genes Expression in Cultured Mammalian Cells
18. Protein Interaction Technologies