Project Z3
Microscopy core facility
Methods in microscopy
Theory and basics of fluorescence microscopy
Date: 27th January 2011
Time: 9.30 am – 16.00 pm
Place: Seminarraum Neubau (Biochemie)
Speakers: Dr. Helge Schmidt (Olympus Deutschland GmbH)
Klaus Willeke (Olympus Deutschland GmbH)
9:30 - 12:30h
Part1: Basic in fluorescence microscopy
- Nature of light: What is resolution? What are airy discs?
- Shining fluorescence: Why use fluorescence?
- Principles: Jablonski-Diagramm, Stockes-Shift, fluorochromes
- Requirements for a fluorescence microscopy system
Lunch break
13:30 - 16:00h
Part 2: Practical Part – “hands on”
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