Proteolysis as a Regulatory Event in Pathophysiology

Sonderforschungsbereich 877

An der Christian-Albrechts-Universität zu Kiel

Research Area Z

(Central Service Projects)

 Z1 (visit project website)
Rose-John (Biochemisches Institut, CAU Kiel)
Central Services
 Z2 (visit project website)
Tholey / Janßen / Leippe (Institut für Experimentelle Medizin, CAU Kiel, Institut für Immunologie, CAU Kiel; Zoologisches Institut, CAU Kiel)
Proteomics platform for the analysis of proteolysis-induced protein alterations and modifications
 Z3 (visit project website)
Saftig / Grötzinger (Biochemisches Institut, CAU Kiel):
Microscopy core facility and antibody production: Analysis of protease structure, cell biology and function

Overview

In addition to the administration and central tasks project (Z1), we propose two service projects, which will be needed by most projects within the SFB initiative. The project Z2, run by Tholey (Institute of Experimental Medicine, University of Kiel), Janßen (Institute of Immunology, University of Kiel) and Leippe (Institute of Zoology, University of Kiel) bundles the expertise of three groups and offers a comprehensive set of state-of-the-art equipment and technologies for differential proteome analysis of peptides and intact proteins using MALDI-TOF/TOF or LC-ESI-MSMS. Thus, the platform provides the expertise for the analysis of protease cleavage sites and a wide range of posttranslational modifications. Together with the application of established methods within the projects of the CRC such as 2- dimensional difference gel electrophoresis (DIGE) and a mass spectrometry-based protein identification service, the platform will also develop novel approaches for the proteome-wide investigation of proteolytic activities. The project Z3 will be coordinated by Saftig and Grötzinger (Institute of Biochemistry, University of Kiel). It will provide a service of monoclonal antibody generation. In addition, state of the art imaging techniques including total life cell imaging laser scan microscopy will be supplied by the Z3 project. A life cell imaging laser scan microscope has recently been approved by the Deutsche Forschungsgemeinschaft through a HBFG proposal.