Prof. Dr. rer. nat. A. Tholey
Instit für Experimentelle Medizin, CAU Kiel
Born 31. 12. 1968
| 1989–1995 | Studies of Chemistry, Univ. d. Saarlandes, Saarbrücken |
| 1995–1998 | PhD-thesis, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg |
| 1999–2002 | PostDoc, Univ. d. Saarlandes, Saarbrücken |
| 2003–2008 | Research Associate, Univ. d. Saarlandes, Saarbrücken |
| 2004 | Research Stage at the “Laboratoire de la Spectrometrie de Masse Bio-Organique” at the Université Louis-Pasteur / CNRS, Strasbourg, (France) |
| 2007 | Habilitation for Analytische Biochemie; Univ. d. Saarlandes, Saarbrücken |
| 2008 | W2-Professor for “Systematische Proteomforschung”, CAU Kiel |
10 Selected Publications
- Melchior K, Tholey A, Heisel S, Keller A, Lenhof HP, Meese E, Huber CG. (2009) Comprehensive proteomic study of human glioblastoma multiforme tissue employing complementary two-dimensional liquid chromatography- mass spectrometry-based approaches. J Proteome Res, in press.
- Lasaosa M, Jakoby T, Delmotte G, Huber CG, Heinzle E, Tholey A. (2009) Rapid selection of peptide containing fractions in off-line two-dimensional HPLC in shotgun proteome analysis by screening with MALDI TOF MS. Proteomics, in press.
- Hussong R, Gregorius B, Tholey A, Hildebrandt A. (2009) Highly accelerated feature detection in proteomics data sets using modern graphics processing units. Bioinformatics 25: 1937-43.
- Selevsek N, Rival S, Tholey A, Heinzle E, Heinz U, Hemmingsen L, Adolph HW. (2009) Zinc ion-induced domain organization in metallo-beta-lactamases: A flexible “zinc arm” for rapid metal ion transfer? J Biol Chem 284: 16419-16431
- Delmotte N, Lasaosa M, Tholey A, Heinzle E, vanDorsselaer A, Huber CG. (2009) Repeatability of peptide identifications in shotgun proteome analysis employing off-line 2Dchromatographic separations and ion-trap tandem mass spectrometry. J Sep Sci 32: 1156-1164
- Lasaosa M, Delmotte N, Melchior K, Heinzle E, Huber CG, Tholey A. (2009) A twodimensional reversed-phase x ion-pair reversed-phase HPLC MALDI TOF/TOF MS approach to shotgun proteome analysis. Anal Bioanal Chem 393: 1245-1256
- Delmotte N, Lasaosa M, Tholey A, Heinzle E, Huber CG. (2007) Two-dimensional reversedphase x ion-pair reversed-phase HPLC: an alternative approach to high-resolution peptide separation for shotgun proteome analysis. J Proteome Res 6: 4363-4373
- Selevsek N, Tholey A, Liénard BRM, Heinzle E, Heinz U, Adolph HW, Frère JM, Oldham NJ, Schofield CJ. (2006) Studies on ternary metallo-ß-lactamase-inhibitor complexes using electrospray ionization mass spectrometry. J Am Soc Mass Spectrom 17: 1000-1004
- Tholey A, Zabet-Moghaddam M Heinzle E, (2006) Quantification of peptides for the monitoring of protease catalyzed reactions by MALDI mass spectrometry using ionic liquid matrixes. Anal Chem 78: 291-297
- Tholey A, Toll H, Huber CG (2005) Separation and detection of phosphorylated and nonphosphorylated peptides in liquid chromatography-mass spectrometry using monolithic columns and acidic or alkaline mobile phases. Anal Chem 77: 4618-4625
