Abstract:
A large number of short tandem repeat (STR) markers spanning the entire human X chromosome have been described and established for use in forensic genetic testing. Due to their particular mode of inheritance, X-STRs often allow easy and informative haplotyping in kinship analyses. Moreover, some X-STRs are known to be tightly linked so that, in combination, they constitute even more complex genetic markers than each STR taken individually. As a consequence, X-STRs have proven particularly powerful in solving complex cases of disputed blood relatedness. However, valid quantification of the evidence provided by X-STR genotypes in the form of likelihood ratios requires that the recombination rates between markers are exactly known. In a collaborative family study, we used X-STR genotype data from 401 two- and three-generation families to derive valid estimates of the recombination rates between 12 forensic markers widely used in forensic testing, namely DXS10148, DXS10135, DXS8378 (together constituting linkage group I), DXS7132, DXS10079, DXS10074 (linkage group II), DXS10103, HPRTB, DXS10101 (linkage group III), DXS10146, DXS10134 and DXS7423 (linkage group IV). Our study is the first to simultaneously allow for mutation and recombination in the underlying likelihood calculations, thereby obviating the bias-prone practice of excluding ambiguous transmission events from further consideration. The statistical analysis confirms that linkage groups I and II are transmitted independently from one another whereas linkage groups II, III and IV are characterised by inter-group recombination fractions that are notably smaller than 50%. Evidence was also found for recombination within all four linkage groups, with recombination fraction estimates ranging as high as 2% in the case of DXS10146 and DXS10134.

Abstract:
OBJECTIVES: The pathogenesis of rectal prolapse (RP) defined by a circumferential, full-thickness invagination of the rectal wall into the anal canal is controversial. RP is normally encountered in elderly women and attributed to several etiological factors (e.g., advanced age, pudendal nerve injury, laxity of supporting ligaments). RP affecting young male patients is unlikely to be explained by these factors and may be due to a rectal motility disorder. Therefore, the enteric nervous system (ENS) as key regulator of intestinal motility was evaluated by a systematic morphometric analysis. PATIENTS AND METHODS: Full-thickness rectosigmoid specimens obtained from young male patients with symptomatic RP (n = 5) and male controls (n = 15) were processed for conventional histology and immunohistochemistry using anti-HuC/D as pan-neuronal marker. Enteric ganglia, nerve and glial cells were quantified separately in the myenteric (MP) and submucosal plexus (SMP). RESULTS: Compared to controls, patients with RP showed significantly (p < 0.05) increased mean ganglionic area both in MP and SMP, increased mean neuronal content of submucosal ganglia, and nearly threefold higher frequency of submucosal ganglia containing ≥7 neurons. CONCLUSION: The morphometric analysis reveals distinct quantitative alterations of the ENS in young male patients with RP mainly characterized by submucosal hyperganglionosis similar to histopathological features described in intestinal neuronal dysplasia. The data give evidence that RP in this unusual subgroup is associated with morphological changes of enteric ganglia which may contribute to the development of RP and complement established etiological concepts.

Abstract:
Objectives: The main goals of this in vitro study were to evaluate the influence of thermocycling, ultrasonic stress and the removal force preload on the retrievability of cemented implant crowns using a clinical removal device (Coronaflex) and evaluating the tensile strength using a universal testing machine (UTM). Methods: Thirty-six crowns were cast from a Co-Cr alloy for 36 tapered titanium abutments (5° taper, 4.3 mm diameter, 6 mm height, Camlog, Germany). The crowns were cemented with a glass-ionomer (Ketac Cem) or a polycarboxylate (Durelon) cement, followed by 3 days of storage in ionized water without thermocycling or 150 days of storage with 37,500 thermal cycles between 5°C and 55°C. Before removal, the crowns were subjected to ultrasonic stress for 0, 5 or 10 min with a contact pressure of either 50 or 500 g. The Coronaflex was used with a removal force preload of 50 or 400 cN, respectively, applied on the point of loading. Scanning electronic microscopy (SEM) was used to evaluate the impact of the removal on the abutment screws. Results: Crowns cemented with the glass-ionomer cement were significantly easier to remove with the Coronaflex or the UTM than crowns cemented with the polycarboxylate cement (P≤0.05). Ultrasonic stress showed no significant impact on the retrievability regardless of the contact pressure or duration applied (P>0.05). No significant differences could be found for both cements when removed with the Coronaflex or the UTM (P>0.05) after thermocycling was applied. A removal force preload of 400 cN resulted in significantly reduced removal attempts in comparison with 50 cN for both cements (P≤0.05). Conclusions: Ultrasound and thermal cycling did not result in reduced cement strength, but to retrieve the crowns, the full impact of a removal instrument has to be applied. Ketac Cem can be used as a "semipermanent" solution, whereas Durelon might serve for permanent cementation. None of the abutment screws showed signs of wear caused by the removal process. To cite this article: Mehl C, Harder S, Schwarz D, Steiner M, Vollrath O, Kern M. In vitro influence of ultrasonic stress, removal force preload and thermocycling on retrievability of implant retained crowns. Clin. Oral Impl. Res. xx, 2011; 000-000. doi: 10.1111/j.1600-0501.2011.02236.x.