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Press release No. 391/2013, 2013-12-18 | zur deutschen Fassung | print version | Search

Great attachment on slippery ground

Scientists of Kiel University analyse attachment in flowing waters


It’s a well-known problem: When biofilm grows on river stones and pebbles, they become extremely slippery. Scientists of Kiel University have discovered that for small animals, this effect is reversed. The so-called biofilm, a slimy film consisting of algae, bacteria and other organic matter, provides insects and their larvae with additional hold. Especially in flowing waters such as streams and riverson this is decisive for the colonization of smooth substrates exposed to strong currents. These findings were published in the renowned scientific magazine „Journal of the Royal Society Interface“, on December 18, 2013.

A series of tests with the larvae of the freshwater mayfly Epeorus assimilis have proven that the biofilm is not only important for the insects as a source of food, but also in questions of attachment. The team of scientists, consisting of bio-physicist Alexander Kovalev and biologists Stanislav Gorb, Jan Michels, Jochen Koop and Petra Ditsche, have found that, in contrast to humans or vehicles, insects are able to apply their attachment mechanisms on slippery biofilm. Even though the larvae’s claws are not able to cling to primary smooth surfaces, they can penetrate the slimy biofilm and interlock their claws with organisms inside of the biofilm. Moreover, the high viscosity of the biofilm strengthens the insects’ ability to resist the current. “It’s like sticking your feet in honey”, explains Dr. Petra Ditsche, member of the work group Functional Morphology and Bio-Mechanics at Kiel University.

The method:
In the laboratory, artificial substrates with various levels of roughness were produced – ranging from extremely smooth to extremely rough. The scientists let biofilm grow over a subset of these substrates. Following this process, the overgrown substrates were compared with the bare substrates of the same type. The surface structure was measured by confocal laser scanning microscopy (CLSM) and analysed to quantify roughness. Indentation measurements were used to determine the biophysical characteristics of biofilm, such as elasticity and hardness. The larvae’s ability to hold onto the substrate was tested in an artificial flume. This test determined the maximum speed at which the larvae were able to cling to a certain kind of substrate. Further, the claws’ retention force (such as friction and clamping) was tested. For this purpose, the scientists fixed specimens of dissected claws on a specific force sensor. The claws were oriented in a natural position and pulled parallel to the particular substrate by a micromanipulator, while forces were recorded.

The findings:
These tests have shown that it is important to consider the biofilm when analysing attachment in aquatic environments because it changes the characteristics of substrates considerably. In the case of the mayfly larvae examined, the attachment forces were stronger on substrates covered with biofilm. That was particularly the case with substrates that were smooth, as well as the ones that displayed only slight roughness. All of them did not provide sufficient surface irregularities for interlocking of the claw. For this reason biofilm should be taken into consideration as a relevant factor influencing attachment on a surface. This applies not only to sessile organisms but also for mobile animals.


Relevant applications
Moreover, these findings can inform future work in other fields, such as antifouling research or the development of artificial attachment devices for underwater purposes.

Photos/material is available for download:
Please pay attention to our ► Hinweise zur Verwendung

Click to enlarge

SEM image of the tarsal claw of E assmilis fore leg. Photo/Copyright: Petra Ditsche

Image to download:
www.uni-kiel.de/download/pm/2013/2013-391-1.jpg

Click to enlarge

Underwater image of E assimilis. Photo/Copyright: Petra Ditsche

Image to download:
www.uni-kiel.de/download/pm/2013/2013-391-2.jpg


Further Links:
Work Group „Functional Morphology and Biomechanics“, at the Institute of Zoology:
www.uni-kiel.de/zoologie/gorb/

Journal of the Royal Society Interface:
http://rsif.royalsocietypublishing.org/content/11/92/20130989.abstract?sid=a6abf5c6-cac7-4b8a-a885-248162daec60

Contact:
Dr. Petra Ditsche
Friday Harbor Laboratories
University of Washington
620 University Road
Friday Harbor, WA, 98250
USA
phone: +1-360-298-2284
Email: pditsche@UW.edu, pditschekuru@zoologie.uni-kiel.de

Prof. Dr. Stanislav Gorb
Functional Morphology and Biomechanics
Institute of Zoology
Christian-Albrechts-Universität zu Kiel
Am Botanischen Garten 1-9
24118 Kiel
phone.: +49 (0) 431/880 4859
Email: sgorb@zoologie.uni-kiel.de

Original publication:
Ditsche P, Michels J, Kovalev A, Koop J, Gorb S. 2014 More than just slippery: the impact of biofilm on the attachment of non-sessile freshwater mayfly larvae. J. R. Soc. Interface 20130989.



Kiel University
Press, Communication and Marketing, Dr. Boris Pawlowski
Address: D-24098 Kiel, phone: +49 (0431) 880-2104, fax: +49 (0431) 880-1355
E-Mail: ► presse@uv.uni-kiel.de
Text / Redaktion: Sebastian Maas