MUTPRED

The program MUTPRED generates mutability profiles of amino 
acid sequences from the corresponding cDNA sequence. The procedure
is described in detail in Cooper and Krawczak (1990). The program
generates UNWEIGHED profiles as described, and WEIGHED profiles 
for which the relative mutabilities are multiplied by the 
likelihood of clinical detection depending on chemical differences 
(Grantham, 1974).

The program will run on all PCs or compatibles with a graphic 
adapter (VGA, EGA, CGA) installed. If the program fails to run on
your computer, you may try to run the program EMUCGA.COM in advance.
MUTPRED does not produce any hardcopies. To get a hardcopy, use
the PRINT SCREEN key on your computer. Alternatively, the <S>ave 
option allows one to download the mutabilities actually displayed 
on the screen in a user defined file. You may then use your own
graphic software to transform these data into a profile. 

MUTPRED is initialized by the file MUTPRED.MSG which has to be 
installed in the current directory. After the program has been 
initialized successfully, MUTPRED asks for the name of the cDNA 
sequence file. This file should contain the plain sequence 
information in the correct reading frame, including nothing else 
than spaces and the four characters A, C, T and G. You may include 
comment lines starting with a semicolon ';'. After a few moments, the 
profile appears on the screen. Strike the '+'  or '-' keys to scroll 
the profile, 'u' or 'w' to generate unweighed or weighed profiles, 
respectively. Strike 'f' to load a new file, 's' to download data, 
'e' to finish.

Best wishes
MK